diff --git a/pkgs/applications/science/biology/picard-tools/default.nix b/pkgs/applications/science/biology/picard-tools/default.nix
index 8fff296affa6..5a9cb5de528e 100644
--- a/pkgs/applications/science/biology/picard-tools/default.nix
+++ b/pkgs/applications/science/biology/picard-tools/default.nix
@@ -2,11 +2,11 @@
 
 stdenv.mkDerivation rec {
   pname = "picard-tools";
-  version = "2.20.8";
+  version = "2.21.1";
 
   src = fetchurl {
     url = "https://github.com/broadinstitute/picard/releases/download/${version}/picard.jar";
-    sha256 = "01bibkwyp4xzwcpvkr3ab6z0syjmzj4zmyxl3bybmrp9irhjvydg";
+    sha256 = "0knfx0by7rml19kr5ynb7860iykij1z1mx2hx0bg3s287sld1ppl";
   };
 
   nativeBuildInputs = [ makeWrapper ];