diff --git a/pkgs/applications/science/biology/trf/default.nix b/pkgs/applications/science/biology/trf/default.nix
new file mode 100644
index 000000000000..f36788eb612c
--- /dev/null
+++ b/pkgs/applications/science/biology/trf/default.nix
@@ -0,0 +1,21 @@
+{ lib, stdenv, fetchFromGitHub }:
+
+stdenv.mkDerivation rec {
+  pname = "trf";
+  version = "4.09.1";
+
+  src = fetchFromGitHub {
+    owner = "Benson-Genomics-Lab";
+    repo = "trf";
+    rev = "v${version}";
+    sha256 = "sha256-73LypVqBdlRdDCblf9JNZQmS5Za8xpId4ha5GjTJHDo=";
+  };
+
+  meta = with lib; {
+    description = "Tandem Repeats Finder: a program to analyze DNA sequences";
+    homepage = "https://tandem.bu.edu/trf/trf.html";
+    license = licenses.agpl3Plus;
+    maintainers = with maintainers; [ natsukium ];
+    platforms = platforms.unix;
+  };
+}
diff --git a/pkgs/top-level/all-packages.nix b/pkgs/top-level/all-packages.nix
index ecd3ce9900a8..3aef21557971 100644
--- a/pkgs/top-level/all-packages.nix
+++ b/pkgs/top-level/all-packages.nix
@@ -36881,6 +36881,8 @@ with pkgs;
 
   treemix = callPackage ../applications/science/biology/treemix { };
 
+  trf = callPackage ../applications/science/biology/trf { };
+
   trimal = callPackage ../applications/science/biology/trimal { };
 
   truvari = callPackage ../applications/science/biology/truvari { };